Product Advantages

• Supports high cloning efficiency and robust colony formation for a variety of cell lines such as: B16F-10, BaF/3, BHK-1, CHO-DG44, CHO-K1, CHO-S, FD-5, HEK-293, Jurkat Daudi, K562, Molt-4, UT-7

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ClonaCell™-TCS Medium is a serum-containing methylcellulose-based semi-solid medium that can be used for selecting and cloning a variety of suspension-adapted cell lines, including CHO-S and hybridomas. It can also be used for semi-solid cloning of some cell lines that grow adherently in the presence of serum, including CHO-K1, BHK-1, and HEK-293. This medium contains pre-selected fetal bovine serum (FBS) and bovine serum albumin (BSA) and supports robust growth of a wide variety of cell types. ClonaCell™-TCS Medium does not contain selection agents. Benefits of semi-solid cloning: Individual cells are suspended in viscous medium and form physically separated, discrete colonies that are easily isolated. Monoclonal cell lines are isolated in less time using fewer resources compared with selection and cloning by limiting dilution. Diverse clones with a wide range of growth rates and productivities form discrete colonies in the viscous medium. As a result, rare and high-producing clones can be individually isolated more easily using simultaneous selection and cloning in semi-solid medium compared with selection in bulk liquid cultures. CONTAINS IMDM (Iscove's Modified Dulbecco's Medium) Methylcellulose Pre-selected serum Bovine serum albumin 2-Mercaptoethanol Phenol red L-Glutamine Other ingredients SUBTYPE 专业培养基, 半固体培养基 CELL TYPE CHO细胞, HEK-293, Other, 杂交瘤 SPECIES 小鼠, 其他 APPLICATION 半固体克隆, 细胞培养 BRAND ClonaCell AREA OF INTEREST 抗体制备, 杂交瘤生成, 细胞系制备